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Cells were washed three times with PBS, lysed in 200 l 0.5% Triton X-100 for 5 min, diluted with 800 l PBS and plated on TSA

and the 6 questions version (IIEF-6) addressing exclusively penile erectile function, but considering the whole spectrum of erection related aspects, including erection frequency, erection firmness, penetration ability, erection maintenance frequency, erection maintenance ability, and erection confidence [30, 31]

Doxorubicin strongly induce plasma levels of Aspartate Aminotransferase, AST, an established indicator of liver damage (Damodar et al., 2014)(Fig

The selective effects of DSIP on the blood supply to the brain explain at least part of this effect